Generation of thick and elastic tissue that is like real tissue but grown from cultured cells has proved difficult with conventional technologies.
Dr. Koichi Nakayama focused on the natural and intrinsic features of cell self-aggregation and invented a novel method to assemble cellular aggregates (spheroids) into any desired three-dimensional macroscopic tissue without collagen or hydrogel materials.
Sphere-like cell aggregates are secured in place by skewers.
The aggregates are allowed to fuse on the skewers for a few days.
The skewers are removed and the tissue is allowed to further mature.
Isolate cells and proliferate them to target levels. In low adhesion multi-well plates, several tens of thousands of cells form a spheroid in a day or two with a diameter of approximately 500 micrometers.
An example for low adhesion multi-well plates: PrimeSurface®96U (MS-9096UZ, S-BIO, Sumitomo Bakelite Co., Ltd.)
Design a three-dimensional arrangement of cellular spheroids and prepare 3D data using the original software. Multiple types of spheroids can be selected as desired.
To design three dimensional structure, we offer special software called “B3D”, which was developed for Regenova.
You can install B3D into your own computer and design no matter where you are.
Needles are aligned to serve as a temporary scaffold during cellular spheroid assembly. The needles are made of stainless steel with a diameter of 100-200 micrometers and pitch of 300-400 micrometers.
Dec, 16, 2015 | 2015 cell biology ascb annual meeting on December 12 to 16, 2015
Dec, 10, 2015 | World Stem Cell Summit 15 on December 10 to 12, 2015
Oct, 17, 2015 | Neuroscience 2015 on Oct 17 to 21, 2015
Sep, 17, 2015 | NIH Research Festival Exhibit on 17th – 18th September, 2015
Jul, 27, 2015 | 3D design software called B3D is newly developed for Regenova